Ming, last time we discussed mouse sperm cryopreservation. How is embryo cryopreservation different, and how should I choose between them? Embryo cryopreservation involves superovulating female mice, mating them with target males, collecting 2-cell embryos, performing slow freezing, and storing them in -196°C liquid nitrogen. The choice depends on recovery considerations and cost factors. Let's explore this in detail.

Ming, for animal experiments, how is anesthesia performed? General or local? What anesthetics are used? In animal research, mouse anesthesia is a critical step for ensuring the smooth progress of experiments. Selecting appropriate anesthetics and methods not only improves efficiency but also minimizes animal distress. Today, let's delve into the details of mouse anesthesia.

Ming, what are the common drug administration methods for mice? Today, let's explore the common injection methods, key operational points, and precautions for mice administration, helping you become more proficient in experimental work!

Ming, the experiment is finished. How should mice be euthanized? What are the methods? The goal of euthanasia is to end an animal's life in the most humane way possible, minimizing pain, fear, and distress. It ensures loss of consciousness and pain perception in the shortest time. Let's discuss the relevant knowledge in detail.

Ming, I often observe various unusual behaviors in mice during breeding. What could be causing these? Laboratory mice sometimes exhibit abnormal behaviors, which may indicate underlying health issues, environmental stress, or effects of experimental interventions. Let's take a detailed look at these "strange" behaviors in research mice today.