Traditional Technology
 

The generation of transgenic mouse models through pronuclear microinjection of DNA constructs into single-cell embryos. The complete process, from construct design to the establishment of a stable line, typically requires 3-4 months.

 

Our services begin with consulting on transgene design. Following construct assembly by the investigator, the Core processes the DNA by excising the transgene from the plasmid backbone and purifying it for microinjection. Concurrently, we manage the production of one-cell-stage embryos through superovulation protocols and prepare pseudopregnant recipient females.

 

The purified DNA is microinjected over a two-day period. Subsequently, the viable embryos are surgically transferred to surrogate mothers. Offspring are screened for transgene integration via polymerase chain reaction (PCR) analysis of genomic DNA isolated from tail biopsies. Founder animals are then bred to establish a transgenic cohort for phenotypic analysis.

 

MingCeler TurboMice™ Technology：Meet the Future of Mouse Model Creation

At Mingceler, we specialize in accelerating genetic research through our TurboMice™ technology, which harnesses optimized tetraploid complementation to deliver fully homozygous mouse models in just 2–4 months—60% faster than traditional breeding methods. Unlike conventional approaches that require 6–12 months of tedious breeding cycles, our process starts with precision editing of embryonic stem cells (ESCs) for targeted gene knockouts, floxing, or multi-locus modifications. We then use tetraploid complementation to develop these ESCs directly into experiment-ready mice, skipping the need for chimeric F1 and F2 generations. This method ensures 100% genotype certainty from the F0 generation, eliminating the guesswork of heterozygous screening and saving researchers thousands of hours spent on colony management.