A Powerful Tool for Elucidating Disease Mechanisms
 

Conditional knockout (cKO) mice are widely utilized to investigate gene-specific functions and model human diseases. In contrast to conventional knockout (KO) models, cKO mice enable targeted gene deletion restricted to particular tissues or induced at specific time points. This approach offers superior spatiotemporal control over gene inactivation, resulting in more physiologically relevant models for studying human disease pathogenesis.
 

Key Applications of Conditional Knockout Mice
 

cKO mice are indispensable for deciphering gene function, elucidating disease mechanisms, and validating compound specificity. These models allow precise manipulation of target gene expression in vivo, facilitating the assessment of drug efficacy and the identification of potential off-target effects.
 

One of the major advantages of conditional knock out is its ability to study gene function in a more controlled manner. By selectively inactivating genes at specific time points or in specific tissues, researchers can observe the consequences of gene loss and gain insights into the underlying mechanisms of various biological processes.

Generation of Conditional Knockout Mice
 

 

 

Utilizing TurboMice™ technology, MingCeler Biotechefficiently generate homozygous cKO mouse models with enhanced success rates. Our optimized gene-editing protocols enable the screening of edited embryonic stem cells within 3–5 days, followed by the construction of tetraploid embryos. Through embryo transfer and surrogate gestation, homozygous cKO mice can be delivered within 2–4 months, reducing the overall timeline by 7–8 months compared to conventional methods.