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NBSGW mouse

Applications in the fields of hematopoietic stem cell transplantation, humanized immune system recon
  • Strain Name:
    NBSGW mouse
  • Product Code:
    M60027
  • Strain Background:
    C57BL/6N
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  • Basic Information
  • Data Validation
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Strain Description
The NBSGW mouse model is a severely immunodeficient mouse strain constructed by combining several key genetic modifications, including a NOD background, SCID mutation, a gain-of-function mutation in the Kit gene (W41, c-Kit W41), and knockout of the IL-2 receptor gamma chain (IL2rg) gene. Its name is derived from its genetic features: NOD background, SCID mutation, c-Kit W41 mutation, and IL2rg deletion. A key advantage of this model is that the c-Kit W41 mutation reduces the competitive niche of endogenous mouse hematopoietic stem cells, while the IL2rg deletion results in profound combined immunodeficiency affecting both innate and adaptive immunity. This combination allows NBSGW mice to efficiently support the engraftment, expansion, and multilineage differentiation of human CD34+ hematopoietic stem cells (HSCs) without the need for toxic myeloablative preconditioning such as total body irradiation (TBI) or chemotherapeutic agents, enabling high-level, long-term, and stable reconstitution of human hematopoiesis and the immune system. This model significantly reduces the reliance on radiation and its associated side effects seen in traditional humanized models, improving experimental standardization and reproducibility. It serves as an advanced platform for studying human hematopoiesis, immunity, blood diseases, and immunotherapy.
  • qWhy not CRISPR or ES Targeting microinjection?
    a
    While CRISPR and ES Targeting microinjection are valuable genetic engineering techniques, TurboMice™ TCT offers distinct advantages. CRISPR can sometimes lead to off - target effects, where unintended genetic changes occur. TCT, on the other hand, provides a more precise way of generating genetically modified mice, minimizing such risks. ES Targeting microinjection often results in chimera mosaic animal animals, meaning different cells in the animal have different genetic make - ups. This can complicate data interpretation and require a lot of breeding and screening. TCT, in contrast, produces mice that are 100% entirely derived from the genetically modified ES cells, ensuring a high level of genetic consistency. Moreover, TCT's ability to rapidly generate homozygous models without extensive breeding sets it apart from these traditional methods, saving both time and resources in the research process. In summary, MingCeler's TurboMice™ TCT offers a unique combination of precision, speed, and viability in genetic engineering, making it the ideal choice for researchers aiming to advance their studies in genetics, pharmacology, and disease modeling.
  • qDoes TurboMice™ Tetraploid Complementation Technology (TCT) produce viable mice?
    a
    Yes, TurboMice™ TCT produces highly viable mice. The mice generated through this technology are genetically stable and fully fertile. The genetic stability ensures that the desired genetic modifications are maintained across generations. Their full fertility means that breeding programs can be easily continued. This is crucial as it allows researchers to expand their mouse colonies for long - term research needs. Whether it's for continuous drug testing, long - term disease progression studies, or large - scale genetic experiments, the viability of the TCT - produced mice provides a reliable and sustainable resource.
  • qHow fast is TurboMice™ Tetraploid Complementation Technology (TCT)?
    a
    TurboMice™ TCT is incredibly fast. Many researchers can obtain homozygous models in just 2 - 4 months. This eliminates the need for the time - consuming initial breeding processes that are typical in other methods. With TCT, you can quickly move from the genetic modification stage to having a ready - to - use homozygous model. This rapid turnaround allows researchers to start their pilot studies sooner. For example, they can begin with male - only pilot studies right after getting the homozygous male mice. Then, they can easily scale up to full - scale experiments using both male and female mice, significantly accelerating the overall research timeline.
  • qWhat is TurboMice™ Tetraploid Complementation Technology (TCT)?
    a
    TurboMice™ Tetraploid Complementation Technology (TCT) is a cutting - edge method in genetic engineering. It combines the power of tetraploid embryo complementation with advanced gene - editing techniques. TCT uses ES cells (embryonic stem cells) which are genetically modified to carry the desired genetic alterations. These modified ES cells are then introduced into tetraploid embryos. The tetraploid cells mainly contribute to the extra - embryonic tissues, while the ES cells develop into the entire mouse embryo. This results in mice that are almost entirely derived from the genetically modified ES cells, ensuring a high degree of genetic uniformity and precision in the final animal model.
  • qHow much do mouse model generation projects cost?
    a
    •Request a free quote.
    •Mingceler specializes in customized mouse model generation services, with project pricing determined by the complexity of the project, materials supplied by the customer, and specified deliverables.
    •We provide a complimentary, no-obligation project design and proposal tailored to your specific requirements.​
  • qHow long does it take to generate a mouse model?
    a
    • Mingceler delivers novel mouse models in an average of 24 weeks.​​​​
    • From scratch, we deliver experiment-ready homozygous target mice (F0) in as fast as 12 weeks.​​​​
    • Please see our timelines for more information.
    Mingceler continuously optimizes project timelines through Lean management principles—stabilizing, standardizing, and refining each process. Our proprietary TurboMice™ technology exemplifies this refinement, enabling rapid generation of genetically engineered mice with industry-leading efficiency while significantly enhancing animal welfare.​
  • qHow soon can Mingceler start your project?
    a
    •Zero project backlog​​ — Mingceler’s streamlined pipeline enables immediate project initiation.
    ​​•Execution commences within 24 hours​​ of receiving signed contract and purchase order.
  • qWhat technology does Mingceler use?
    a
    •Mingceler utilizes proprietary ​​Turbomice™ technology​​ for embryonic stem (ES) cell gene targeting, ​​significantly enhancing 3R compliance​​ and ​​accelerating development timelines​​ for genetically engineered mouse models.
    •Developed through systematic optimization of ​​tetraploid complementation​​ and ​​stem cell precision editing​​, TurboMice™ enables ​​targeted genomic modifications at virtually any locus​​ within ​​3–5 months​​.
  • qWhat genetic backgrounds are offered?
    a
    Mingceler offers gene targeting on the following backgrounds:
    •C57BL/6N
    •BALB/c
    •ICR
    Less common strains as required
  • qDoes Mingceler offer partial mouse model projects?
    a
    Mingceler recommends end-to-end services from project design to homozygous mouse delivery, ensuring integrated quality control (QC) for ES cell targeting and germline transmission. For clients seeking flexible or partial solutions, we provide comprehensive modular services—including custom ES cell bank establishment—and leverage our TurboMice™ technology for rapid model generation:

    • Custom ES cell bank creation (tailored to client-specific genetic backgrounds or targeting needs)
    • Gene targeting vector construction
    • Edited ES cell derivation (using Mingceler or client-supplied vectors)
    • TurboMice™-based mouse line generation (from client/repository ES cells or custom ES banks)
    Rapid delivery advantage: Once clients request model production from stored ES cells, we complete the ES-to-mouse process in approximately 45 days.
    • Validation and phenotyping services (for all models)
  • qDoes Mingceler sell off-the-shelf models?
    a
    • QuickMice™ nuNPG Mice
    • QuickMice™ HPV Mice
    • QuickMice™ hHLA Mice
    • QuickMice™ LMNA Mice
    • QuickMice™ hPSCK9 Mice
    • QuickMice™ hTIGIT Mice
    • QuickMice™ hACE2 Mice
  • qWhat kind of humanised models does Mingceler generate?
    a
    •Complete Human Gene Replacement
    Replace entire mouse genes with human counterparts while retaining native murine promoters
    Enables physiological human gene expression patterns in mouse
    •Multi-Gene Locus Humanization
    Simultaneous replacement of multiple contiguous genes
    Capable of handling fragments up to several hundred kilobases per project
    •Domain Humanization
    Targeted replacement of specific functional domains (e.g., extracellular domains)
    Preserves mouse gene architecture while introducing human functional elements
    •Point Mutation Humanization
    Replace specific mouse gene nucleotides with human equivalents
    Ideal for modeling single-nucleotide polymorphisms (SNPs) and disease-causing mutations
  • qHow much for a project assessment?
    a
    •Free initial design proposal with zero obligations.​
    •Request a free quote!
  • qWho owns the mouse lP? Do l need a licence from Mingceler?
    a
    All model generation projects of Mingceler operate under a fee-for-service framework.
    IP Ownership: All intellectual property rights related to custom mouse models, including derived organs, tissues, cells, and biological materials, are the sole and exclusive property of the Client.
    Third-Party Transfer Permission: The Client may independently decide to retain, utilize, or commercialize their custom models project materials (e.g., targeting vectors, ES cells, mouse lines) without the need for prior consent from Mingceler.
    Licensing Exemption: The Client has full autonomy over all uses of the custom models or their derivatives, including but not limited to commercialization, distribution to third parties, and publication involving model data. No written license from Mingceler is required for such uses.

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