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hBDCA2 mouse

  • Strain Name:
    C57BL/6NMcl-Rosa26tm1(hBDCA2)/MCL
  • Product Code:
  • Strain Background:
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  • Basic Information
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Strain Description
The human BDCA2 gene and its promoter are inserted into the Rosa26 safe site in mice.

Dendritic cells, as the most powerful professional antigen-presenting cells, serve as the "bridge" connecting innate immunity and adaptive immunity. Among the many subpopulations of dendritic cells, plasmacytoid dendritic cells (pDCs) can produce interferon type I extremely efficiently, thereby directly inhibiting viral replication and activating various immune cells such as natural killer cells, T lymphocytes, and myeloid dendritic cells, coordinating the entire antiviral immune response. However, autoimmune diseases such as systemic lupus erythematosus and inflammatory diseases lead to the continuous activation of pDCs, causing dysfunction of pDCs, producing a large amount of interferon type I, forming an "interferon type I signature," driving autoimmune reactions, and attacking self-tissues. Moreover, in specific tumor microenvironments, pDCs may exhibit functional tolerance or dysfunction, failing to effectively initiate antitumor immunity and instead possibly promoting immune suppression through the induction of regulatory T cells and other means, aiding in the immune escape of tumors [1]. Therefore, pDCs have become an important target for the treatment of autoimmune diseases, chronic inflammation, and cancer.

Blood dendritic cell antigen 2 (BDCA-2, also known as CD303, CLEC4C) is a member of the type II transmembrane C-type lectin family, consisting of a type II transmembrane glycoprotein composed of 213 amino acids. It is selectively expressed on the surface of pDCs in humans and non-human primates. Additionally, BDCA-2 is a functional immunoreceptor [2]. When BDCA-2 is cross-linked by its specific antibody or ligand, it transmits inhibitory signals to the interior of pDCs. This signal can recruit molecules containing an immunoreceptor tyrosine-based inhibitory motif through its associated FcRγ chain, thereby inhibiting the production of interferon type I and pro-inflammatory factors mediated by the TLR7/9 signaling pathway [3].
  • Data Validation





  • qGame-changing benefits？
    a
    While competitors highlight germline efficiency gains, shorter timelines and enhanced 3Rs animal welfare benefits for their technologies, these are merely incremental improvements over traditional approaches. In sharp contrast, our proprietary technology delivers fully pure, homogeneous lineages—every single cell of the mice is derived exclusively from totipotent ES cells, with guaranteed 100% germline transmission efficiency. To experience these unparalleled benefits firsthand, enquire about your custom mouse model project with us or order embryos for in-house validation at your facility.
  • qWhy not CRISPR or ES Targeting microinjection?
    a
    While CRISPR and ES Targeting microinjection are valuable genetic engineering techniques, TurboMice™ TCT offers distinct advantages. CRISPR can sometimes lead to off - target effects, where unintended genetic changes occur. TCT, on the other hand, provides a more precise way of generating genetically modified mice, minimizing such risks. ES Targeting microinjection often results in chimera mosaic animal animals, meaning different cells in the animal have different genetic make - ups. This can complicate data interpretation and require a lot of breeding and screening. TCT, in contrast, produces mice that are 100% entirely derived from the genetically modified ES cells, ensuring a high level of genetic consistency. Moreover, TCT's ability to rapidly generate homozygous models without extensive breeding sets it apart from these traditional methods, saving both time and resources in the research process. In summary, MingCeler's TurboMice™ TCT offers a unique combination of precision, speed, and viability in genetic engineering, making it the ideal choice for researchers aiming to advance their studies in genetics, pharmacology, and disease modeling.
  • qDoes TurboMice™ Tetraploid Complementation Technology (TCT) produce viable mice?
    a
    Yes, TurboMice™ TCT produces highly viable mice. The mice generated through this technology are genetically stable and fully fertile. The genetic stability ensures that the desired genetic modifications are maintained across generations. Their full fertility means that breeding programs can be easily continued. This is crucial as it allows researchers to expand their mouse colonies for long - term research needs. Whether it's for continuous drug testing, long - term disease progression studies, or large - scale genetic experiments, the viability of the TCT - produced mice provides a reliable and sustainable resource.
  • qHow fast is TurboMice™ Tetraploid Complementation Technology (TCT)?
    a
    TurboMice™ TCT is incredibly fast. Many researchers can obtain homozygous models in just 2 - 4 months. This eliminates the need for the time - consuming initial breeding processes that are typical in other methods. With TCT, you can quickly move from the genetic modification stage to having a ready - to - use homozygous model. This rapid turnaround allows researchers to start their pilot studies sooner. For example, they can begin with male - only pilot studies right after getting the homozygous male mice. Then, they can easily scale up to full - scale experiments using both male and female mice, significantly accelerating the overall research timeline.
  • qWhat is TurboMice™ Tetraploid Complementation Technology (TCT)?
    a
    TurboMice™ Tetraploid Complementation Technology (TCT) is a cutting - edge method in genetic engineering. It combines the power of tetraploid embryo complementation with advanced gene - editing techniques. TCT uses ES cells (embryonic stem cells) which are genetically modified to carry the desired genetic alterations. These modified ES cells are then introduced into tetraploid embryos. The tetraploid cells mainly contribute to the extra - embryonic tissues, while the ES cells develop into the entire mouse embryo. This results in mice that are almost entirely derived from the genetically modified ES cells, ensuring a high degree of genetic uniformity and precision in the final animal model.
  • qHow much do mouse model generation projects cost?
    a
    •Request a free quote.
    •Mingceler specializes in customized mouse model generation services, with project pricing determined by the complexity of the project, materials supplied by the customer, and specified deliverables.
    •We provide a complimentary, no-obligation project design and proposal tailored to your specific requirements.​
  • qHow long does it take to generate a mouse model?
    a
    • Mingceler delivers novel mouse models in an average of 24 weeks.​​​​
    • From scratch, we deliver experiment-ready homozygous target mice (F0) in as fast as 12 weeks.​​​​
    • Please see our timelines for more information.
    Mingceler continuously optimizes project timelines through Lean management principles—stabilizing, standardizing, and refining each process. Our proprietary TurboMice™ technology exemplifies this refinement, enabling rapid generation of genetically engineered mice with industry-leading efficiency while significantly enhancing animal welfare.​
  • qHow soon can Mingceler start your project?
    a
    •Zero project backlog​​ — Mingceler’s streamlined pipeline enables immediate project initiation.
    ​​•Execution commences within 24 hours​​ of receiving signed contract and purchase order.
  • qWhat technology does Mingceler use?
    a
    •Mingceler utilizes proprietary ​​Turbomice™ technology​​ for embryonic stem (ES) cell gene targeting, ​​significantly enhancing 3R compliance​​ and ​​accelerating development timelines​​ for genetically engineered mouse models.
    •Developed through systematic optimization of ​​tetraploid complementation​​ and ​​stem cell precision editing​​, TurboMice™ enables ​​targeted genomic modifications at virtually any locus​​ within ​​3–5 months​​.
  • qWhat genetic backgrounds are offered?
    a
    Mingceler offers gene targeting on the following backgrounds:
    •C57BL/6N
    •BALB/c
    •ICR
    Less common strains as required
  • qDoes Mingceler offer partial mouse model projects?
    a
    Mingceler recommends end-to-end services from project design to homozygous mouse delivery, ensuring integrated quality control (QC) for ES cell targeting and germline transmission. For clients seeking flexible or partial solutions, we provide comprehensive modular services—including custom ES cell bank establishment—and leverage our TurboMice™ technology for rapid model generation:

    • Custom ES cell bank creation (tailored to client-specific genetic backgrounds or targeting needs)
    • Gene targeting vector construction
    • Edited ES cell derivation (using Mingceler or client-supplied vectors)
    • TurboMice™-based mouse line generation (from client/repository ES cells or custom ES banks)
    Rapid delivery advantage: Once clients request model production from stored ES cells, we complete the ES-to-mouse process in approximately 45 days.
    • Validation and phenotyping services (for all models)
  • qDoes Mingceler sell off-the-shelf models?
    a
    • QuickMice™ nuNPG Mice
    • QuickMice™ HPV Mice
    • QuickMice™ hHLA Mice
    • QuickMice™ LMNA Mice
    • QuickMice™ hPSCK9 Mice
    • QuickMice™ hTIGIT Mice
    • QuickMice™ hACE2 Mice
  • qWhat kind of humanised models does Mingceler generate?
    a
    •Complete Human Gene Replacement
    Replace entire mouse genes with human counterparts while retaining native murine promoters
    Enables physiological human gene expression patterns in mouse
    •Multi-Gene Locus Humanization
    Simultaneous replacement of multiple contiguous genes
    Capable of handling fragments up to several hundred kilobases per project
    •Domain Humanization
    Targeted replacement of specific functional domains (e.g., extracellular domains)
    Preserves mouse gene architecture while introducing human functional elements
    •Point Mutation Humanization
    Replace specific mouse gene nucleotides with human equivalents
    Ideal for modeling single-nucleotide polymorphisms (SNPs) and disease-causing mutations

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